I mentioned the "HistoLogic" article here some months ago. We love the 
Alcian yellow/toluidine blue, but you can't find alcian yellow dye anymore. 
We're currently trying to find a way to stretch out the use of our last 
bottle of alcian yellow solution. As for the Gimenez stain we found it very 
hard to get the proper results, and the pathologists said that they 
definately preferred the AY/TB techique for readability.

Tara Oakes,HT


>From: Phil Bergin 
>To: Rachel Stoat , "histonet@pathology.swmed.edu" 
>
>Subject: Re: H. pylori stains
>Date: Thu, 08 Nov 2001 15:25:18 +0100
>
>Hi Rachel
>
>There are a couple of posts on pylori stains in the archives one:
>     stains for Helicobacter pylori 3.5K Aug 9, 2000
>     
>http://www.histosearch.com/histonet/Aug00/stainsforHelicobacterpylo.html
>             by               RSRICHMOND@aol.com
>
>has an article reference comparing :  A comparison of staining methods for
>Helicobacter pylori. Kim
>Rhatigan-Drexler, MA, HTL (ASCP), histology supervisor, SUNY. In 
>Histo-Logic,
>April first, 1999.
>This study examined a single gastric biopsy specimen, stained with six
>methods: Giemsa, Gimenez' carbol fuchsin-malachite green, a Dieterle
>microwave method, cresyl echt violet, Sayeed's methylene
>blue-PAS-hematoxylin, and toluidine blue-Alcian yellow. Nice 
>photomicrographs
>illustrate each stain.
>
>Although I'd be interested in any more, especially if they also stain 
>H.felis
>and work in mice.  Good luck and hope it helps.
>
>Phil
>
>
>Rachel Stoat wrote:
>
> > Hello!
> >
> > I'm looking for special stains other than Giemsa, Warthin-Starry or tol.
> > blue to stain for helicobacter pylori.  Can anyone help?
> >
> > Thanks,
> >
> > Rachel
> >
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