Re: Frozen sections

From:Phil Bergin


This might be a stupid question...but are you drying and fixing the sections?
We cut our frozen sections, place them on superfrost slides, allow them to
airdry for up to 8 hrs, then fix in 100% icecold acetone 4-8 min (I know there
are lots of fixing procedures out there!).  This seems to hold the sections on
the slides really well for us.


Department of Medical Microbiology and Immunology
Göteborg University
Box 435
405 30 Göteborg

"Miller, Barbara" wrote:

> Hello all,
> I have just started working with frozen sections ( I had previously just
> worked with paraffin sections) and I am having a problem with
> sections floating off the slide when I wash them to remove the OCT.  Will
> warming the slides help the sections to adhere to the slides?
> I am using superfrost plus slides so I wasn't expecting this problem.  Does
> anyone out there have any suggestions or ideas?
> Thank you in advance for your help, you always come through for me.
> Bobbi Miller
> Department of Gastroenterology
> Roudebush VA Medical Center
> Indianapolis, IN 46202
> Fax  1-317-554-0116
> e-mail

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