Re: Freezing tissues previously formalin fixed
--- "Pasquetto, Silvia"
wrote: > Hi guys!
> I need your help again.
> In our lab we perform oil red o' stain on livers,
> frozen immediately after
> necropsy of animals in a hexane cooled ice bath.
> Recently, our pathologists required that this
> freezing should be perform
> after formalin fixation.
> I found an old procedure regarding a passage in a
> dextrane solution of
> tissues before freezing.
> Using this procedure, when I tried to cut the
> samples at the cryostat, the
> tissues crumbled and was impossible obtain a decent
Try rinsing the Formalin fixed tissue in water, place
in a 20% OCT (or similar freezing support material)and
place on a rotator for 1 hour, remove tissue and
gently blot dry, freeze on a chuck as per usual,
section and place sections on sticky slides (APTS
etc). Stain as per usual.
Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
The Childrens Hospital at Westmead
Locked Bag 4001
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