Re: Freezing tissues previously formalin fixed

From:Nancy E Weber

Hi Silvia,
When I needed to do a frozen section on previously fixed samples, I usually
did not do anything else to them.  I'd gross a section out and freeze it in
OCT on the chuck in the cryostat and never had much trouble getting a usable

Nancy Weber
Research Associate
Veterinary Clinical Sciences
Ohio State University
----- Original Message -----
From: "Pasquetto, Silvia" 
Sent: Friday, November 09, 2001 11:05 AM
Subject: Freezing tissues previously formalin fixed

> Hi guys!
> I need your help again.
> In our lab we perform oil red o' stain on livers, frozen immediately after
> necropsy of animals in a hexane cooled ice bath.
> Recently, our pathologists required that this freezing should be perform
> after formalin fixation.
> I found an old procedure regarding a  passage in a dextrane solution of
> tissues before freezing.
> Using this procedure, when I tried to cut the samples at the cryostat, the
> tissues crumbled and was impossible obtain a decent slice!
> Any suggestion would be greatly appreciated.
> Thanks in advance
> Silvia
> GSK Company
> Histopatology Lab

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