Re: Freezing paraffin sections before IHC
Dear Jane
the theory is that freezing the slides inhibits oxidation, the same premise
as meat(yes meat). I have studied this quite extensively and have found this
to be so about a great many antibodies. Elias published sort of on this in ER
and Pr and I have found it to be quite true in Ki-67, EGFR, and others. try
it for yourself, one stored at room temp one at -20, another at 4 degrees and
try storing for 1 week , 2 weeks etc, you will see the antigenicity slope
down or get weak, this is how I have tested. Good Luck
dana
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