You can try albuminized slides, heat works sometimes. The plus slides are
for formalin fixed tissue only as I understand. I have found that like
paraffin slides, a well cut section stays on better. Try a new blade, it
might help some.

-----Original Message-----
From: Miller, Barbara [mailto:bmiller2@iupui.edu]
Sent: Wednesday, October 31, 2001 2:49 PM
To: 'histonet@pathology.swmed.edu'
Cc: Miller, Barbara
Subject: Frozen sections


Hello all,

I have just started working with frozen sections ( I had previously just
worked with paraffin sections) and I am having a problem with
sections floating off the slide when I wash them to remove the OCT.  Will
warming the slides help the sections to adhere to the slides?
I am using superfrost plus slides so I wasn't expecting this problem.  Does
anyone out there have any suggestions or ideas?

Thank you in advance for your help, you always come through for me.

Bobbi Miller
Department of Gastroenterology
Roudebush VA Medical Center
Indianapolis, IN 46202

Fax  1-317-554-0116

e-mail   bmiller2@iupui.edu