RE: FITC or DIG?
Thank you Ralph!
I thought about biotin but the thing is most tissue I am going to do is
Thanks again for your suggestion!
>From: "Ralph Marcucio"
>To: "'Carol Chang'"
>Subject: RE: FITC or DIG?
>Date: Wed, 14 Nov 2001 11:37:21 -0800
>I would suggest biotinylating your antibody since it will allow you to
>amplify your reaction using streptavidin etc. I think Sigma sells a
>biotinylkating reagent called NHS-long chain biotin. It is fairly easy to
>put the biotin on the antibody and get rid of any unreacted biotin.
>Ralph Marcucio, PhD
>From: Carol Chang [mailto:email@example.com]
>Sent: Wednesday, November 14, 2001 10:50 AM
>Subject: FITC or DIG?
>I need to use a human antibody on human tissue. So I am going to label the
>antibody either with DIG or with FITC. Could anybody tell me which one is
>better? And which anti-DIG or anti-FITC antibody works best? The histonet
>the best. It helped me a lot.
>Thanks in advance.
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