Carol-

I would suggest biotinylating your antibody since it will allow you to
amplify your reaction using streptavidin etc.  I think Sigma sells a
biotinylkating reagent called NHS-long chain biotin.  It is fairly easy to
put the biotin on the antibody and get rid of any unreacted biotin.

Ralph Marcucio, PhD

-----Original Message-----
From: Carol Chang [mailto:carolc50@hotmail.com]
Sent: Wednesday, November 14, 2001 10:50 AM
To: histonet@pathology.swmed.edu
Subject: FITC or DIG?


Hello:
I need to use a human antibody on human tissue.  So I am going to label the
antibody either with DIG or with FITC. Could anybody tell me which one is
better? And which anti-DIG or anti-FITC antibody works best? The histonet is
the best. It helped me a lot.
Thanks in advance.
Carol

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