RE: FITC or DIG?
I would suggest biotinylating your antibody since it will allow you to
amplify your reaction using streptavidin etc. I think Sigma sells a
biotinylkating reagent called NHS-long chain biotin. It is fairly easy to
put the biotin on the antibody and get rid of any unreacted biotin.
Ralph Marcucio, PhD
From: Carol Chang [mailto:firstname.lastname@example.org]
Sent: Wednesday, November 14, 2001 10:50 AM
Subject: FITC or DIG?
I need to use a human antibody on human tissue. So I am going to label the
antibody either with DIG or with FITC. Could anybody tell me which one is
better? And which anti-DIG or anti-FITC antibody works best? The histonet is
the best. It helped me a lot.
Thanks in advance.
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