RCC antibody

From:mward@wfubmc.edu (Martha Ward)

Martha Ward wrote:

I am having trouble working up the antibody renal cell carcinoma marker
(RCC).  I have a renal cell carcinoma for a control and I use a Ventana
ES and reagents.  I tried the antibody with and without AB blocker
because I was getting as much staining in my negative as I was in my
antibody slide.  The AB blocker worked well but I am not getting any
signal.  I tried citric acid as well as EDTA for a retrieval solution
and tried it in a pressure cooker as well as microwave.  If anyone has
any suggestions I would be grateful for any and all.  Thanks!!

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