Cryoprotect fixed liver in 15% sucrose not dextran, just place tissue in
this solution, and let it sink to bottom of container, overnight at 4C.
  
Blot excess 15% sucrose off tissue, snap freeze per your protocol, section.

The sucrose replaces the water in formalin, gives better freezing, better
sectioning. 

There has been a great deal of discussion on cryoprotection on Histonet, go
to Archive for a search.


Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)