ORO on formalin fixed liver

From:Gayle Callis

Cryoprotect fixed liver in 15% sucrose not dextran, just place tissue in
this solution, and let it sink to bottom of container, overnight at 4C.
Blot excess 15% sucrose off tissue, snap freeze per your protocol, section.

The sucrose replaces the water in formalin, gives better freezing, better

There has been a great deal of discussion on cryoprotection on Histonet, go
to Archive for a search.

Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)

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