I'm doing a Fluorescein to double labeling on BrDu and hAAT and secondary on
FITC and Texas Red, afterward. However, I have never done a nucleus
staining. In the end I need to count cells that stain for my primary
antibody and cells that do not.
Can anyone tell me or give me a protocol to stain a nucleus after my
secondary for my primary antibody? What and where can I purchase a reagent
for staining a nucleus?
Many thanks if you can help.
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