Nucleus staining

From:Thu-Thao Pham

Hi all,

I'm doing a Fluorescein to double labeling on BrDu and hAAT and secondary on 
FITC and Texas Red, afterward. However, I have never done a nucleus 
staining. In the end I need to count cells that stain for my primary 
antibody and cells that do not.

Can anyone tell me or give me a protocol to stain a nucleus after my 
secondary for my primary antibody? What and where can I purchase a reagent 
for staining a nucleus?

Many thanks if you can help.


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