Lectin controls

From:Gayle Callis

This was given to me by Vector Tech services, go to their catalog and see
the specificity guide for lectins, fucose was correct for the UEA.    

Negative control is dilute UEA (0.5 mg/ml, 1:200 (a concentration I have
used) in 200 to 500 mM fucose in buffer, let stand for 30 min at Room
temperature, add this to your section.  Fucose + UEA = negative since UEA
has bound to the fucose. I did not use the lectin buffer (a TRIS buffered
saline with extra Mg and Ca) but UEA and PNA worked well with Dulbeccos PBS
containing 0.05% Tween 20 (do not exceed 0.1% Tween).  I avoided additional
serums or BSA in all my diluents per advice of Vector.  Lectins are sticky!  

For other's information, Vector has a wonderful specificity chart for all
the lectins, identifying the sugars you need to use as a negative control
protocol for the particular lectin you are working with.

Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)

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