GMA recovery - sucess!
|From:||"Renton, Lousie, Mrs" <firstname.lastname@example.org>|
*** Delete now if you are not interested in plastic embedding or my
Thank you for all your advice and information on recovering my
precious samples. I am so pleased that I thought that I would
share my sucess story with everyone. ]
My samples were in cassettes in a glass jar with approximately
200mls of GMA infiltrating soln. This is the 1st time that I have
used this particular product - so what follows can perhaps be
blamed on ignorance.
The samples were left under vacuum at RT for about 10 days - by
which time they had solidified into a single mass. So, acting on
advice received I first donned relevant personal protective gear and
using Percussive technology (hammer) directed a few judiciuios
blows to jar. The whole mass was then soaked in alcohol ( I used
absolute- others recommend 95%). After a few days the mass was
softened enough to loosen apart the glued together cassettes and
rescue individual biosies which I then further cleaned off by digital
They are now soaking in the fridge in a 1:1 soln of infiltrating soln
and ethanol - further to infiltrating for a SHORT time and embedding.
Thank you once again for all your help and advice.
Bone research Unit
<< Previous Message | Next Message >>