----- Original Message -----
From: "Jan Shivers" 
To: "Johnson, Theresa" 
Sent: Friday, November 02, 2001 9:53 AM
Subject: Re: IHC question for research folks


> Teri,
>
> I have used DAKO's smooth muscle actin antibody (clone 1A4) successfully
on
> mouse tissue, with the DAKO ARK kit.  I did NOT have success using the
usual
> cytokeratin markers on mouse tissue (clones Lu-5, MNF116, 34BE12, NCL5D3,
> OV-TL 12/30, Ks20.8).  I had to go to antibodies made specifically against
> mouse tissue in order to get any staining.  These anti-mouse CK antibodies
> were obtained by one of the researchers, and I don't believe they are
> commercially available.
>
> Jan Shivers
>
> ----- Original Message -----
> From: "Johnson, Theresa" 
> To: "Histonet (E-mail)" 
> Sent: Friday, November 02, 2001 9:32 AM
> Subject: IHC question for research folks
>
>
> > I sent this to the VIR email list and I don't think it's gone out yet,
so
> > let me query the histonet folks.
> >
> > When given a choice of host animals for primary antibodies, I find
myself
> > shying away from the mouse monoclonals, looking for other host species,
> > especially rabbit.  I know there are various MOM kits available, and my
> > question relates to whether I should be bold and try them anyway, or
> > continue to search for alternate sources of whatever antibody I'm
looking
> > for.
> >
> > Specifically, at this time, I'm looking a pan-cytokeratins and muscle
> > specific actins, to use as positive control for my detection system when
> > developing new antibodies in mouse.
> >
> > Thanks for any help!
> >
> > Teri Johnson
> > Manager Histology Core Facility
> > Stowers Institute for Medical Research
> > 1000 E. 50th St.
> > Kansas City, Missouri  64110
> > tjj@stowers-institute.org
> >
> >
> >
>