Freezing tissues previously formalin fixed
I need your help again.
In our lab we perform oil red o' stain on livers, frozen immediately after
necropsy of animals in a hexane cooled ice bath.
Recently, our pathologists required that this freezing should be perform
after formalin fixation.
I found an old procedure regarding a passage in a dextrane solution of
tissues before freezing.
Using this procedure, when I tried to cut the samples at the cryostat, the
tissues crumbled and was impossible obtain a decent slice!
Any suggestion would be greatly appreciated.
Thanks in advance
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