Freezing paraffin sections before IHC

From:Jane Radford

Hi Histonetters,
I have recently read an article that described sectioning paraffin 
embedded blocks and drying in an oven as normal (40 degrees C), but 
then freezing the sections overnight at -20 degrees C before 
immunohistochemical staining the next day. The antigen in question 
was CD11b.
I hoping someone may be able to shed some light on what the freezing 
step achieves.
Thank you in advance for your help.

Jane Radford

Jane Radford
Senior Technical Officer
Department of Pathology
University of Sydney
NSW Australia
Ph: 61 2 9351 6152

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