> ----------
> From: 	Monson, Frederick C.
> Sent: 	Wednesday, November 14, 2001 3:37 PM
> To: 	'Dr. Reinhard von Wasielewski'
> Subject: 	RE: image analyzers for Ki-67 stainings
> 
> Try ImageJ (URL:  http://rsb.info.nih.gov/ij/)  or one of derivatives of
> its antecedent NIH Image (URL:
> http://rsb.info.nih.gov/nih-image/index.html)  such as Object Image (URL:
> http://simon.bio.uva.nl/object-image.html).  Then perhaps search "particle
> counting" or "nuclear counting" on Google.  BUT, it would appear that this
> may have been done already:  [Anal Quant Cytol Histol 2001
> Jun;23(3):218-28, "Computerized image analysis of Ki-67 in ductal breast
> carcinoma in situ", Menter DG, Hoque A, Motiwala N, Sahin AA, Sneige N,
> Lieberman R, Lippman SM., Departments of Clinical Cancer Prevention and
> Pathology, University of Texas M. D. Anderson Cancer Center, Houston,
> Texas, USA.]. 
> 
> Hope this helps,
> 
> Fred Monson
> 
> Frederick C. Monson, PhD 
> Center for Advanced Scientific Imaging(CASI) 
> West Chester University of Pennsylvania 
> Schmucker Science Center II  
> South Church Street                                                    
> West Chester, PA, 19383
> eMail:  fmonson@wcupa.edu
> http://darwin.wcupa.edu/casi/
> 
> 
> 
> ----------
> From: 	Dr. Reinhard von Wasielewski
> Sent: 	Wednesday, November 14, 2001 2:13 PM
> To: 	histonet@pathology.swmed.edu
> Subject: 	image analyzers for Ki-67 stainings
> 
> Dear Histonetters,
> I am looking for the optimal computer program and camera hardware to 
> start with image analyzing of immunostainings. We have some thousand 
> stainings of breast cancer I want to (or better: I do not want to) 
> count to get an idea of proliferation on a percentual basis. 
> Who has good experience with this issue ?
> We use blue counterstain and AP as enzyme (Fast Red / New Fuchsin).
> Many thanks in advance.
> Reinhard von Wasielewski
> Reinhard von Wasielewski, MD
> 
> 
>