FW: image analyzers for Ki-67 stainings
|From:||"Monson, Frederick C." |
> From: Monson, Frederick C.
> Sent: Wednesday, November 14, 2001 3:37 PM
> To: 'Dr. Reinhard von Wasielewski'
> Subject: RE: image analyzers for Ki-67 stainings
> Try ImageJ (URL: http://rsb.info.nih.gov/ij/) or one of derivatives of
> its antecedent NIH Image (URL:
> http://rsb.info.nih.gov/nih-image/index.html) such as Object Image (URL:
> http://simon.bio.uva.nl/object-image.html). Then perhaps search "particle
> counting" or "nuclear counting" on Google. BUT, it would appear that this
> may have been done already: [Anal Quant Cytol Histol 2001
> Jun;23(3):218-28, "Computerized image analysis of Ki-67 in ductal breast
> carcinoma in situ", Menter DG, Hoque A, Motiwala N, Sahin AA, Sneige N,
> Lieberman R, Lippman SM., Departments of Clinical Cancer Prevention and
> Pathology, University of Texas M. D. Anderson Cancer Center, Houston,
> Texas, USA.].
> Hope this helps,
> Fred Monson
> Frederick C. Monson, PhD
> Center for Advanced Scientific Imaging(CASI)
> West Chester University of Pennsylvania
> Schmucker Science Center II
> South Church Street
> West Chester, PA, 19383
> eMail: firstname.lastname@example.org
> From: Dr. Reinhard von Wasielewski
> Sent: Wednesday, November 14, 2001 2:13 PM
> To: email@example.com
> Subject: image analyzers for Ki-67 stainings
> Dear Histonetters,
> I am looking for the optimal computer program and camera hardware to
> start with image analyzing of immunostainings. We have some thousand
> stainings of breast cancer I want to (or better: I do not want to)
> count to get an idea of proliferation on a percentual basis.
> Who has good experience with this issue ?
> We use blue counterstain and AP as enzyme (Fast Red / New Fuchsin).
> Many thanks in advance.
> Reinhard von Wasielewski
> Reinhard von Wasielewski, MD
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