FW: a sample prep queri HELP

From:Andrea Grantham

I do a lot of ovaries here - A LOT, but they are usually rodent and are 
fixed in 10% formalin, Bouins or 70% ETOH not tequila (what a waste of good 
tequila - assuming that it was of the good variety). I hope that the 
ovaries were small or trimmed before they were plunked into the tequila.
I'd process them mostly out of curiosity to see how they might turn out. 
The ultrastructure wouldn't be much to speak of but there might be some 
histology to see. One reply on the microscopy listserver stated that 
tequila is about 40% alcohol. If so I'd trim the samples if necessary and 
process as I usually do by starting in 70% ETOH through 80%, 95% x2, 100% 
x3, Xylene x 2, paraffin x 2.
The replier on microscopy also said to rehydrate in PBS and refix in 
formalin. You could do that. In fact this might be a good idea it might 
help to preserve what hasn't already been destroyed.
I hope Rosemary processes the wolf ovaries and gives a report on the outcome.
Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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