Decal with heat

From:"Cazares, Ruth"

Hi all,

I was wondering if anyone out there is decalcifying large bone specimens
with heat (femoral heads for example)?
According to literature, heat is not recommended since the loss of calcium
salts occurs very fast and swelling and hydolysis of the bone occurs
interfering with certain stains. But I'm sure there are ways around this,
especially just for H&E staining.
 How is this being done? I would appreciate any information on this subject
that would help accelerate the turn-around time on these 
specimens.


	
Respectfully,
								
								Ruth Cazares
								




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