Conditional immortalization

From:Sara Kalla

Well, first off, since I'm new, I'll introduce myself.  I work in Dr.
Harry Blair's lab at the University of Pittsburgh.  We're looking at bone
turnover, and the processes of osteoblast and osteoclast differentiation.
I'm new in the lab, and fresh out of college (an ecology major to boot),
so I suspect I'll be asking you guys a lot of questions.

Anyways, here's my first question:
We want to do a conditional immortalization on monocytes from an
osteopetrotic patient.  I've found some information on using a temperature
sensitive mutant of the large t-antigen of SV40 (pZipSVtsa58), but it's
old (10+ years) and I haven't been able to find the vector.  Is there an
newer/better way of going about it?  Any information at all would be

Sara Kalla

774 Scaife Hall
Dept. of Pathology
U. of Pittsburgh

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