|From:||Bruce Abaloz <email@example.com>|
HI - I AM A PhD STUDENT & AM HOPING SOMEONE CAN OFFER ADVICE/METHOD. I cannot make a good quality smear slide!! Could you please give me some
idea how to make a smear slide or get a good method to do that from your
What I have done to make the slide is the following:
the concentration of the final cell suspension is about 3-6 x 10(6)/ml, 5
ul of the cell suspension was dropped on the slide and smeared gently to a
small circular area, then air dry and fixed in acetone for 10 min. finally,
the fixed slide was stored in -80 degree freezer.
But the quality of the slide is not satisfactory for immunocytochemistry.
THANKYOU VERY MUCH........
Zhen Zhang!! (ZZ)
DEPARTMENT of ZOOLOGY * ph: +61 3 83446282
The UNIVERSITY of MELBOURNE * fax: +61 3 83447909
Parkville, Victoria.3010 * email: firstname.lastname@example.org
**NOTE: Changed postcode and telephone (9344.... becomes 8344....)**
OPEN THE MIND;STRENGHTHEN THE BODY;FORTIFY THE SPIRIT!!
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