HI  - I AM A PhD STUDENT  & AM HOPING SOMEONE CAN OFFER ADVICE/METHOD.              I cannot make a good quality smear slide!! Could you please give me some
idea how to make a smear slide or get a good method to do that from your
net system.

What I have done to make the slide is the following: 
the concentration of the final cell suspension is about 3-6 x 10(6)/ml, 5
ul of the cell suspension was dropped on the slide and smeared gently to a
small circular area, then air dry and fixed in acetone for 10 min. finally,
the fixed slide was stored in -80 degree freezer. 
But the quality of the slide is not satisfactory for immunocytochemistry.
THANKYOU VERY MUCH........
Zhen Zhang!! (ZZ)

BRUCE ABALOZ
HISTOLOGIST		      
DEPARTMENT of ZOOLOGY	           *  ph:    +61 3 83446282
The UNIVERSITY of MELBOURNE        *  fax:   +61 3 83447909 
Parkville, Victoria.3010           *  email: b.abaloz@zoology.unimelb.edu.au                                                                       
AUSTRALIA.
   **NOTE: Changed postcode and telephone (9344.... becomes 8344....)**                                                                                                                               
         OPEN THE MIND;STRENGHTHEN THE BODY;FORTIFY THE SPIRIT!!                 
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