Re: herceptest antigen retrieval
|From:||Abizar Lakdawalla <email@example.com>|
a pressure cooker would be a better and SAFER bet.
Kappeler Andreas wrote:
> Hi Marg
> Welcome to the club! When we gave the manufacturer of the Herceptest a bad
> time for their protocol (because it has been developed at sea level where
> water boils as close to 100 C as possible) they were not very enthusiastic
> about our comments (I'm stupid enough to live at a higher altitude). We've
> tried almost everything under the sun using waterbaths, but were unable to
> maintain 95 C for the buffer INSIDE the coplin jar, although we managed to
> have this temperature for the waterbath, but that's not the point. Now, as
> we do not live in FDA-country (even though I'm sure that the new president
> of the USA - if there ever will be one - will within short declare FDA
> rules to be the standard for the whole known world, i.e. the USA), we
> modified the manufacturer's protocol and on an on-site inspection, the Dako
> rep approved of our method - and a couple of months later asked for our
> protocol ... Basically we are boiling the slides directly in the buffer,
> using a Bunsen burner (which already gave me a couple of nasty comments on
> the histonet on an earlier occasion). However, if you're living at too high
> an altitude, where water boils at less than 95 C, our method will not solve
> your problem. Insist with the manufacturer that they solve your problem (and
> don't accept sending out the slides to a lab at sea level as a solution).
> I'm including the protocol for you (but not the histonet) as a PDF file.
> Good luck.
> Andi Kappeler
> Institute of Pathology, University of Bern, Switzerland
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