Re: help for IHc stains on whole mount embryos

From:Carrie Kyle <>

One problem would be that he is using a mouse primary on mouse tissue.  Since the secondary is directed against should be expected that the secondary will stick to nearly everything.  Try recommending one of the commercially available kits specifically for this type if IHC.  Both Dako and Zymed have them.
Good luck,
Carrie Kyle-Byrne, HT(ASCP)

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