Hi Nima,

I was just talking with Nancy at Biocare Medical and they have a new CD4 on
the market that they've had great success with.  You can give them a call:
(800)799-9499.  I don't think you can get by without HIER.

Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
Immunohistochemistry/In Situ Hybridization Laboratory
D4/218-2472
600 Highland Avenue
Madison, WI  53792-2472


(608)265-6596
FAX: (608)263-1568


-----Original Message-----
From: Nima Farsinejad [mailto:nfarsin@emory.edu]
Sent: Monday, November 27, 2000 10:36 AM
Cc: Histonet
Subject: ANtibody Search: CD4


Hi Histonetters!

	Once again, I am in desperate need of your help. I am trying to
stain CD4 cells in human liver tissue. This tissue has been fixed and
paraffin embedded. I have found many CD4 antibodies out there but they are
all for either Flow Cytometry and/or Frozen Tissue sections. I have found
one from Novocastra that will work with Paraffin embedded tissue, however,
Antigen retrieval is required and I would prefer an antibody that would
not require this step. I would appreciate any help anyone could give me.
Thanks.

Nima Farsinejad
Research Specialist
Digestive Diseases
Emory University
Atlanta, Georgia