Hi Dear Histonetters.
I always couldn't make a good quality smear slide,could you give me some
idea how to make the smear slide or get a good method to do that from your
net system.

What I have done to make the slide is the following: 
the concentration of the final cell suspension is about 3-6 x 10(6)/ml, 5ul of the cell suspension was dropped on the slide and smeared gently to a small circular area, then air dry and fixed in acetone for 10 min, finally,the fixed slide was stored in -80 degree freezer. 
But the quality of the slide is not satisfactory for immunocytochemistry.

Thanks a lot

ZZ
Zhen zhang
Zoology Dept.
University of Melbourne
Parkvelle, 3052
Tel 61-3-8344 4334
Fax 61-3-8344 7909

BRUCE ABALOZ
HISTOLOGIST		      
DEPARTMENT of ZOOLOGY	           *  PH:    +61 3 83446282
The UNIVERSITY of MELBOURNE        *  FAX:   +61 3 83447909 
Parkville, Victoria.3010           *  EMAIL: b.abaloz@zoology.unimelb.edu.au                                                                       
AUSTRALIA.
                    DANCE, LIKE NO - ONE'S WATCHING!!
          YOU ARE A SPIRITUAL BEING HAVING A HUMAN EXPERIENCE!!

                                                                                         This message is confidential;its contents do not constitute a commitment by The University Of Melbourne except where provided for in a written agreement between you and The University Of Melbourne. Any unauthorised disclosure, use or dissemination, either whole or partial, is prohibited. If you are not the intended recipient of the message, please notify the sender immediately.