HELP - PLEASE!!!!
|From:||Bruce Abaloz <email@example.com>|
Hi Dear Histonetters.
I always couldn't make a good quality smear slide,could you give me some
idea how to make the smear slide or get a good method to do that from your
What I have done to make the slide is the following:
the concentration of the final cell suspension is about 3-6 x 10(6)/ml, 5ul of the cell suspension was dropped on the slide and smeared gently to a small circular area, then air dry and fixed in acetone for 10 min, finally,the fixed slide was stored in -80 degree freezer.
But the quality of the slide is not satisfactory for immunocytochemistry.
Thanks a lot
University of Melbourne
Tel 61-3-8344 4334
Fax 61-3-8344 7909
DEPARTMENT of ZOOLOGY * PH: +61 3 83446282
The UNIVERSITY of MELBOURNE * FAX: +61 3 83447909
Parkville, Victoria.3010 * EMAIL: firstname.lastname@example.org
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