The Alpha Smooth Muscle Actin from DAKO (M0851) works excellently on every
species I've tried it on.  I dilute my own antibody preparations, and this
one is used at a 1:200 dilution (for 30 minutes) with an LSAB2 kit on a DAKO
autostainer.

Jan Shivers
University of Minnesota
College of Veterinary Medicine
Veterinary Diagnostic Laboratory

-----Original Message-----
From: Louri Caldwell <louri_c@hotmail.com>
To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
Date: Wednesday, November 29, 2000 1:45 PM
Subject: Smooth Muscle Actin for Veterinary Medicine


>Hi everyone,
>
>I have a researcher who is interested in doing IHC for Smooth Muscle Actin.
>Do you have any suggestions on which antibody works best for Veterinary
>tissue.  I believe she is interested primarily in performing it on feline
>and canine tissue.
>
>Any suggestions you have would be greatly appreciated.
>
>Thanks in advance for your help.
>
>Louri Caldwell
>College of Veterinary Medicine
>University of Georgia
>
>
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