I use polyclonal anti-Myoglobin from DAKO as well and have never had any
trouble with it.  I use it at 1:1000 dilution with no pretreatment.

-----Original Message-----
From: rzumbo <rzumbo@iofm.cs.nsw.gov.au>
To: HISTONET@pathology.swmed.edu <HISTONET@pathology.swmed.edu>
Date: Tuesday, November 21, 2000 5:10 PM
Subject: Myoglobin


Hi all,
Can anyone help me please. We are having some trouble with our
myoglobin antibody, it is a polyclonal from Dako. We are having a
lot of trouble with background staining. We have tried most things
such as diluting the antibody using avidin/biotin blockers. Has
anybody any suggestions.
Thankyou

Rosalba Zumbo
Technical Officer ( Scientific)
Institute of Forensic Medicine
Sydney, Australia
Fax 61 2 95664573