Fab2-FITC secondaries

From:"John C. Dennis" <dennijc@vetmed.auburn.edu>

Dear All:

I was quite pleased with my batch of Fab2-FITC (goat
anti-rabbit) secondary from Boehringer
Mannheim.  Now I'm out.  Roche bought up B/M (in the spirit of leaner and
meaner management style) and Roche no longer sells the antibody.  The
Roche tech rep recommended Chemicon.  The best I could get shines real
green but sticks to the
mouse tissue I'm working on these days (Head and parts thereof).  I use
plenty of normal serum and 5% BSA so I don't think the blocker is the
problem.  I think the antibody is the problem.

Does anyone out there have a favorite anti-rabbit IgG to recommend?

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849

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