|From:||"John C. Dennis" <firstname.lastname@example.org>|
I was quite pleased with my batch of Fab2-FITC (goat
anti-rabbit) secondary from Boehringer
Mannheim. Now I'm out. Roche bought up B/M (in the spirit of leaner and
meaner management style) and Roche no longer sells the antibody. The
Roche tech rep recommended Chemicon. The best I could get shines real
green but sticks to the
mouse tissue I'm working on these days (Head and parts thereof). I use
plenty of normal serum and 5% BSA so I don't think the blocker is the
problem. I think the antibody is the problem.
Does anyone out there have a favorite anti-rabbit IgG to recommend?
John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL 36849
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