1. scrunched gels, 2. gels sticking to antiroll plate

From:Jerry Chi-Yuan Hu <ragnarok@ruf.rice.edu>

Hello Everyone,

One of my friends from a neighboring lab is performing histology on cells
embedded in PEG hydrogels.  She often encounters the problem of the PEG
gel getting pushed by the blade and scrunched up at one end.  I suggested
a lower temperature, and she tried sectioning at -15, -20, and -80 C,
still getting the same problem.  I am guessing that this is due to a
difference between the stiffness of her freezing medium and her PEG gel.  

Has anyone encountered this sort of problem?  Are there softer freezing
mediums she can try out (she is currently using something from Triangle

Also, another strange problem she has been having is that the PEG gel
sometimes gets stuck to the antiroll plate...I have not the faintest clue
what is going on here.

Any help or suggestions will be greatly appreciated!

Jerry Hu

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