collagen fluorescence

From:"Corazon D. Bucana" <>

Hi all,

I would appreciate any information on the availability of eriochrome black
T dye.  I am trying to find a way to suppress fluorescence due to collagen
and I came across a reference that uses this dye as a counterstain so that
an immunofluorescence specimen viewed with FITC filters will show red
collagen fibers.

Also if anyone can suggest a way of suppressing fluorescence of collagen in
frozen sections I would appreciate it very much.


Cora Bucana
Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747

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