TEM sample preparation for knee joints

From:Whitni Babcock <whitnibabcock@hotmail.com>

I am a college student involved in some research work in which I prepare 
mice knee joints for TEM then look at the ultrastructure of the articular 
cartilage.  Currently I use a 3 day standard procedure, but I have heard of 
a microwave prep that could cut the prep time down some keeping the 
structure fresher.  Does anyone know any procedure for that?  Also what 
would be the optimal way to decalcify the bone to make sectioning easier?

I appreciate all suggestions!  Thank you!
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