TEM sample preparation for knee joints
|From:||Whitni Babcock <firstname.lastname@example.org>|
I am a college student involved in some research work in which I prepare
mice knee joints for TEM then look at the ultrastructure of the articular
cartilage. Currently I use a 3 day standard procedure, but I have heard of
a microwave prep that could cut the prep time down some keeping the
structure fresher. Does anyone know any procedure for that? Also what
would be the optimal way to decalcify the bone to make sectioning easier?
I appreciate all suggestions! Thank you!
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