Re: "High" endothelial cells

From:Kappeler Andreas <>

Hi Mark

There is an antibody against a 'cutaneous lymphocyte associated antigen'
(CLA), which reacts with a 200 kD glycoprotein that is preferentially found
on a subset of peripheral T lymphocytes and helps them in 'homing' to the
skin. The same antigen is also found on a subset of CD68+ monocytes,
Langerhans cells as well as on high endothelial venules (HEV) in lymphoid
tissue, however not in vascular denothelia of non-lymphoid tissue. The
antibody may help your pathologist in identifying HEVs in lymph nodes etc.,
but he should expect to see considerable reactivity in other cells as well.
Rat-anti-CLA, clone HECA-452, is available from Pharmingen (Cat #  35821 A).
We use it at 5 ug Ig/ml on FFPE tissue after antigen retrieval with HIER
(citrate buffer, pH 6.0). A rat Ig-specific secondary antibody may be
advantageous, although many anti-mouse Ig antibodies will show sufficient
cross-reactivity with rat Igs (we are using Dako's gt-a-mo Ig/B, Cat #
E0433). Good luck!

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

"High" endothelial cells

> Hello again
> One of our pathologists was asking this morning if there is a way to
> mark the "high" endothelial cells found in lymph nodes and
> lymphatic collections.  The method can be either IHC or
> histochemical.  Any ideas?  We are using archived FFPE human
> tissues.
> Thanks for any input.
> Mark
> W. Mark Elliott, PhD
> Research Associate
> Pulmonary Research Laboratory
> Room 292, St. Paul's Hospital
> 1081 Burrard Street
> Vancouver BC Canada
> V6Z 1Y6
> 604-806-8346
> 604-806-8351 (Fax)

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