Re: collagen fluorescence

From:Lesley Weston <lesley@interchange.ubc.ca>

This sounds interesting - what was the reference? Thanks.

Lesley Weston.



On Tue, 7 Nov 2000, Corazon D. Bucana wrote:

> Hi all,
> 
> I would appreciate any information on the availability of eriochrome black
> T dye.  I am trying to find a way to suppress fluorescence due to collagen
> and I came across a reference that uses this dye as a counterstain so that
> an immunofluorescence specimen viewed with FITC filters will show red
> collagen fibers.
> 
> Also if anyone can suggest a way of suppressing fluorescence of collagen in
> frozen sections I would appreciate it very much.
> 
> Thanks,
> 
> Cora Bucana
> Corazon D. Bucana, Ph.D.
> Dept. Cancer Biology
> UT M.D.Anderson Cancer Center
> 1515 Holcombe Blvd, Box 173
> Houston, Texas 77030
> Phone: 713-792-8106
> FAX 713-792-8747
> 
> 




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