Re: Paraffin Block preparation from Cultured Cells

From:Neuropathology <>

We have use Shandon's Cytoblock with a great deal of success for small cell

Andy Shand

----- Original Message -----
From: C.M. vander Loos <>
To: <>
Sent: Thursday, November 16, 2000 8:08 AM
Subject: RE: Paraffin Block preparation from Cultured Cells

> Carry wrote:
> >Hello!  I am attempting to make paraffin blocks of cultured cells and our
> >pathologist has noted that the cell density is not good enough, so I am
> >asking for tips/ protocols that anyone may be using.
> >
> >I start with at least 6 million cells, pellet them in a 15 ml centrifuge
> >tube, pour off media and add a couple drops of warm 3% Eosin/Agar and
> >to mix.  The cells are immediately chilled on ice and the agar cell
> >is fixed in Formalin and processed on a short cycle on an automated
> >processor.  At this time, we embed the entire processed pellet, but we
> >trying to core a paraffin block and insert a cored agar pellet with no
> >avail.
> >
> >Any tips out there?
> >
> >Carrie
> ************************
> Dear Carry,
> Have a look at HMJ Kerstens et al. Agarcyto: a novel cell-processing
> for multiple molecular diagnostic analysis of the uterine cervix. J
> Histochem Cytochem (2000) 48:709-718. Although they are working with agar
> as well, you may find some additional tips there and his e-mail address.
> Good luck.
> Chris

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