Re: Paraffin Block preparation from Cultured Cells

From:Kenneth Wester <>

Hi Carrie.

Maybe the following two references could give you some hints.

Riera et al. Am J Clin Pathol 111: 329-335, 1999
Wester et al. J Pathol 190: 503-511, 2000


>Hello!  I am attempting to make paraffin blocks of cultured cells and our
>pathologist has noted that the cell density is not good enough, so I am
>asking for tips/ protocols that anyone may be using.
>I start with at least 6 million cells, pellet them in a 15 ml centrifuge
>tube, pour off media and add a couple drops of warm 3% Eosin/Agar and vortex
>to mix.  The cells are immediately chilled on ice and the agar cell pellet
>is fixed in Formalin and processed on a short cycle on an automated
>processor.  At this time, we embed the entire processed pellet, but we are
>trying to core a paraffin block and insert a cored agar pellet with no
>Any tips out there?

Kenneth Wester, Ph.D.
Depts. of Surgical Sciences and Genetics & Pathology
Experimental urology, The Rudbeck laboratory
Uppsala University
Dag Hammarskjöldsväg 20
S-752 37 Uppsala, Sweden
phone:  +46 (0)18 611 02 06
fax:    +46 (0)18 471 3432

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