Re: NeuN antibody

From:Dana Settembre <settembr@UMDNJ.EDU>

On Fri, 3 Nov 2000, Neuropathology wrote:

> Anyone using NeuN antibody on FFPE sections of brain? If so can you tell me
> the supplier of the antibody?
> 
> Thanks
> Bob Quilty
> Dept of Neuropathology
> Frenchay Hospital
> Bristol
> UK
> 
> 
> 

Hello Bob,
I use NeuN from Chemicon, cat.# MAB377.  I use it at a dilution of 1:200
with citra pretreatment of 15 minutes in the microwave.  I'm sure other
steamers, pressure cookers will work too.

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey
USA




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