Re: NeuN antibody

From:Dana Settembre <settembr@UMDNJ.EDU>

On Fri, 3 Nov 2000, Neuropathology wrote:

> Anyone using NeuN antibody on FFPE sections of brain? If so can you tell me
> the supplier of the antibody?
> Thanks
> Bob Quilty
> Dept of Neuropathology
> Frenchay Hospital
> Bristol
> UK

Hello Bob,
I use NeuN from Chemicon, cat.# MAB377.  I use it at a dilution of 1:200
with citra pretreatment of 15 minutes in the microwave.  I'm sure other
steamers, pressure cookers will work too.

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey

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