Re: Mouse Brain Processing

From:Geoff McAuliffe <mcauliff@UMDNJ.EDU>

Peter Poon wrote:

>  We have a problem with tissue processing we hope someone out there can
> help us with. We are formalin-fixing, dehydrating, and wax-infiltrating
> mouse brains in the standard manner for subsequent sectioning and staining.
> Our problem is that the ventricles are greatly expanded. This morpholgical
> distortion is critical, as we are doing quantitative immunohistochemistry
> on the hypothalamus, and a third ventricle that looks like a football (or
> worse) instead of a slit means the tissue lateral to the ventricle is
> distorted and compressed. Any suggestions would be greatly appreciated.
> Thanks
> Peter C. Poon

Dear Peter:

    A few questions first:
how were these brains fixed, immersion or perfusion?
does this condition exist before fixation?
What does the rest of the brain look like, specifically are the cells swollen
or ...?"

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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