Re: GLYOXAL AND IHC
|From:||Mary Bryhan <email@example.com>|
We are a tried and true Anatech customer and use Prefer fixative. We do have some problems from time to time with certain antibodies, but generally do not experience total loss of antigenicity on all antibodies. I too would like to know more about unstable epitopes that are reduced or lost over time, however we frequently go back and perform IHC on older blocks without problem most of the time.
Mary Bryhan HT (ASCP)
Northern Michigan Hospital
>>> Lee & Peggy Wenk <firstname.lastname@example.org> 11/17/00 05:10AM >>>
Need some help again.
Received a call. A histo lab has switched to a glyoxal fixative.
When they first perform an IHC on any of the tissues, for any antibody,
it works great.
If the lab goes back to the same BLOCK a couple of weeks later, and
cuts some more slides, the intensity of the staining is less.
If the labs waits a couple more weeks and cuts more slides from the
BLOCK, there is NO IHC staining.
Yet, the new BLOCKS (just processed) are staining great. So they know
that the antibodies are fine. But wait a couple of weeks on the new BLOCKS,
and the same decrease in staining pattern repeats.
They thought it might be underfixation, so they have increased the
time in fixation, but the pattern is still repeating.
I know about decrease of staining on previously cut/stored SLIDES.
But has anyone experienced this on BLOCKS, for any antibody,
in just a couple of weeks time? Is it a fixation/processing problem
in general, or is it specific to glyoxal fixatives?
Any and all thoughts from the collective minds of Histonet are welcomed.
Peggy A. Wenk, HTL(ASCP)
William Beaumont Hospital
Royal Oak, MI 48073
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