Re: Antigen Retrieval Methods
|From:||Shirley Powell <powell_sa@Mercer.EDU>|
I use Biocare's Decloaking Chamber for most heat retrieval, but I also
have used Cell Prep (from American Biosafety 916-652-8021) at room temp
overnight for some difficult antigens. You might try using whatever
retrieval buffer you have in a water bath at 70 overnight. Also
consider trying no retrieval if you have not already done so. Remember,
what works in one person's hands does not always have success in
another's. I have had success using a highly alkaline household cleaner
for some cases, but will hold my comments on that until further
studies. If you wish to discuss further, call me 478-301-2374.
Daniel Martinez wrote:
> --- Shirley Powell <powell_sa@Mercer.EDU> wrote:
> > Are you having no staining, trouble with sections
> > washing off, or over
> > staining?
> > Daniel Martinez wrote:
> > > I currently have a rare Parkinson's case that
> > I
> > > am having trouble staining. This tissue has been
> > > fixing in formalin for 13yrs. I am attempting to
> > > stain this tissue with several alpha-synuclein
> > > antibodies that we produce. To date, I have tried
> > > formic acid, proteinase-K, microwave, and boiling
> > > treatments. Any advice on other methods that
> > might be
> > > worth trying would be appreciated. Thanks for
> > your
> > > help
> > >
> > > Dan Martinez
> > > CNDR/University of Pennsylvania
> > >
> > > __________________________________________________
> > > Do You Yahoo!?
> > > Thousands of Stores. Millions of Products. All
> > in one Place.
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> Sorry Shirley,
> I have been getting either no staining or
> nonspecific staining.--Dan
> Do You Yahoo!?
> Thousands of Stores. Millions of Products. All in one Place.
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