RE: warthin-starry

From:"Greer, Bonnie" <Bonnie.Greer@stjude.org>

Worthless and sorry....

Bonnie G. Greer, HT (ASCP)
Histology Laboratory Manager
Animal Resources Center
901-495-2272
FAX: 901-495-3112
Pager: 901-495-3578 (#2378)


-----Original Message-----
From: Leek, Adrian [mailto:ALeek@cytologix.com]
Sent: Monday, November 06, 2000 11:41 AM
To: 'Atoska Gentry'
Cc: histonet@pathology.swmed.edu
Subject: RE: warthin-starry


Atoska:
I have not found a way to get rid of the random silver precipitate (I think
the scientific name for it is "crud").  I have found I can avoid it by
putting the sections on plain glass slides (we normally use Plus slides).
With our Warthin-Starry stain, it appears to be a problem confined to a few
BLOCKS, and has nothing to do with the tissue.  When we get it, the "crud"
is fairly uniformly distributed over the area corresponding to the wax
block, extending beyond the edge of the tissue, then stopping fairly
abruptly some distance out.  Indeed, looking at the slide by eye, you can
often see a ghost outline of the block around the tissue.
If anyone else has a solution, I would love to hear it.
Adrian Leek,
CytoLogix Corp.


-----Original Message-----
From: Atoska Gentry [mailto:gentras@vetmed.auburn.edu]
Sent: Friday, November 03, 2000 02:11 PM
To: histonet@pathology.swmed.edu
Subject: warthin-starry


Hello has anyone doing warthin-starry stain experienced excessive black 
dots on the sections in question and on the slide surrounding the 
sections?  If so please I need your help.  What is it the silver 
precipating out and staining the gelatin?  what causes this?  And most 
importantly how do you get rid of it?   I already tried excessive washing 
in hot tap H2O. thanks, Atoska

Atoska  S. Gentry B.S., HT(ASCP)
Research Assistant II
Scott-Ritchey RSCH Center
Auburn University, AL  36849
PH:   (334) 844-5579
Fax:  (334) 844-5850




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