RE: Slides in 20 years

From:"Morken, Tim" <tim9@cdc.gov>

"The recipes dealt with the absence of chloral hydrate."

If I remember correctly Chloral Hydtrate is added as a preservative to
prevent bacterial/fungal growth. It shouldn't affect staining at all.

Tim Morken, BA, EMT(MSA), HTL(ASCP)
Infectious Disease Pathology Activity
Centers for Disease Control and Prevention
Ms-G32
1600 Clifton Road
Atlanta, GA 30333
USA

PH: 404-639-3964
FAX: 404-639-3043

email: tim9@cdc.gov




-----Original Message-----
From: Scott Taft [mailto:ss336@yahoo.com]
Sent: Monday, November 13, 2000 1:38 PM
To: HistoNet Server
Cc: kreinhardt@ventanamed.com
Subject: Slides in 20 years


Histonetters,

I have seen various Mayer's Hematoxylin recipes over
the months posted here. The recipes dealt with the
absence of chloral hydrate. My question is this:

Does anyone know what the staining intensity might be
like in 20 years if the slides are pulled from the
slide files. Especially if they use this for their H &
E stain.

Another way of asking: Has anyone been using this
recipe for awhile and if so, how do earlier slides
look after being pulled from the slide files?

Thanks,
Scott Taft (HT ASCP)
Tucson, AZ

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