RE: PAP / Hydrophobic pens

From:"Morken, Tim" <>

I like the PAP pen and we even use it on the BioGenex Optimax occasionally
to reduce the amount of reagent we need to use for ISH. The only drawback
I've seen is that if you use an aqueous mounting media the PAP pen material
will not be removed prior to coverslipping and can cause some
air-trapping/bubble problems.

Tim Morken, BA, EMT(MSA), HTL(ASCP)
Infectious Disease Pathology Activity
Centers for Disease Control and Prevention
1600 Clifton Road
Atlanta, GA 30333

PH: 404-639-3964
FAX: 404-639-3043


-----Original Message-----
From: Aidan Schurr []
Sent: Monday, November 13, 2000 3:12 PM
Subject: PAP / Hydrophobic pens

Hi all,

We currently do all our immuno's by hand, wiping around sections with
tissues to contain the reagents.  Who out there uses PAP pens or other
hydrophobic markers, and what do you think of them?  I would like to use
them but are put off by the price...

Many thanks,
Aidan, New Zealand.

a i d a n   c   s c h u r r
 section head, histology
  department of pathology
hutt valley health
high street, lower hutt
new zealand
telephone ++64 4 5709173
facsimile ++64 4 5709214

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