RE: Mouse Brain Processing

From:"Barry, Lilith" <>

Are you perfusing the brains? If so then the perfusion speed is too fast.

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-----Original Message-----
From: Peter Poon []
Sent: Thursday, November 09, 2000 12:06 PM
Subject: Mouse Brain Processing

 We have a problem with tissue processing we hope someone out there can 
help us with. We are formalin-fixing, dehydrating, and wax-infiltrating 
mouse brains in the standard manner for subsequent sectioning and staining. 
Our problem is that the ventricles are greatly expanded. This morpholgical 
distortion is critical, as we are doing quantitative immunohistochemistry 
on the hypothalamus, and a third ventricle that looks like a football (or 
worse) instead of a slit means the tissue lateral to the ventricle is 
distorted and compressed. Any suggestions would be greatly appreciated.

Peter C. Poon

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