RE: Mouse Brain Processing
|From:||"Barry, Lilith" <Lilith.Barry@nrc.ca>|
Are you perfusing the brains? If so then the perfusion speed is too fast.
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From: Peter Poon [mailto:firstname.lastname@example.org]
Sent: Thursday, November 09, 2000 12:06 PM
Subject: Mouse Brain Processing
We have a problem with tissue processing we hope someone out there can
help us with. We are formalin-fixing, dehydrating, and wax-infiltrating
mouse brains in the standard manner for subsequent sectioning and staining.
Our problem is that the ventricles are greatly expanded. This morpholgical
distortion is critical, as we are doing quantitative immunohistochemistry
on the hypothalamus, and a third ventricle that looks like a football (or
worse) instead of a slit means the tissue lateral to the ventricle is
distorted and compressed. Any suggestions would be greatly appreciated.
Peter C. Poon
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