RE: Mouse Brain Processing

From:"Saby, Joseph" <>


Can you see this expansion in the cut block face?  If not, then this
artifact may be due to over-expansion of the cut section on the waterbath.
To correct this problem, reduce the temperature of the waterbath and watch
the sections very carefully, picking them up just before they are fully
flattened (they will finish flattening on the slide).


Joseph A. Saby, BA, HT(ASCP)
Drug Safety Evaluation
Pfizer Global Research and Development
2800 Plymouth Road
Ann Arbor, MI 48105
Phone: (734)-622-3631
FAX:   (734)-622-3866

-----Original Message-----
From: Peter Poon []
Sent: Thursday, November 09, 2000 12:06 PM
Subject: Mouse Brain Processing

 We have a problem with tissue processing we hope someone out there can 
help us with. We are formalin-fixing, dehydrating, and wax-infiltrating 
mouse brains in the standard manner for subsequent sectioning and staining. 
Our problem is that the ventricles are greatly expanded. This morpholgical 
distortion is critical, as we are doing quantitative immunohistochemistry 
on the hypothalamus, and a third ventricle that looks like a football (or 
worse) instead of a slit means the tissue lateral to the ventricle is 
distorted and compressed. Any suggestions would be greatly appreciated.

Peter C. Poon

<< Previous Message | Next Message >>