RE: H&E, (and how to get advice)

From:"Circle :>" <>

Dear John and histoners,

You said right. One of my friends from Beijing has this experience of
buying small amount of antibodies. However, there is no such companies in
Hong Kong. Moreover, I can't find any suitable company which provide
antibodies for vascular endothelial cells. My friends also mentioned about
the problem of Factor VIII and its co factor. He cannot get any results
from it. What should I do?

Thank you very much!


On Mon, 6 Nov 2000, Tarpley, John wrote:

> Circle,
> If you want to try immunohistochemistry you might check with the various
> antibody vendors to see if they have samples sizes of antibodies available.
> Sometimes these sizes are available at lower cost or even free. This might
> give you the opportunity to demonstrate the value of IHC to your supervisor
> in order to obtain more funds to continue the project. I agree with John
> Kiernan's point that while an H&E may be useful for normal vessels, you will
> probably find it difficult to distinguish between endothelial cells and
> smooth muscle cells in various pathological conditions.
> John E. Tarpley 5-1-A
> Associate Scientist
> Amgen Inc.
> One Amgen Center Drive
> Thousand Oaks, CA 91320 
> These Opinions are my own and not necessarily those of my employer.
> -----Original Message-----
> From: Circle :> []
> Sent: Monday, November 06, 2000 1:03 AM
> To: J. A. Kiernan
> Cc: Histonet
> Subject: Re: H&E, (and how to get advice)
> Dear John,
> Oh, sorry for any inconvenience. My name is Circle. Sounds strange to
> Westerners, but it's a friendly name in my place. You may called my
> surname Chan.
> The reason why I asked this question is that I borrow a CD-rom from
> library, (called Histology image review, by Frank J. Wilson) which
> contains lots of histology and IHC photos. And I noticed
> that the stain they used is H&E. However, I found the colour is different
> among different slides. I used H&E to counter stain some cells before. The
> technicans told me that H gives the colour of purple/blue (on the
> nucleus) and E gives orange/red (on the cytoplasm). What I found in the
> CD-rom is that I can identify different structures on the slides. For
> example, I can distinguish endothelial cells and the arterial muscle
> cells. That is, they have differnt tone on differnet parts of the tissue!
> Other reason I would like to try on H&E rather than IHC is the price of
> the antibody. I know factor VIII can identify endothelial
> cells. Unfortuantely, my supervisor has already rejected as the antibody
> cost much. 
> I have the alpha-actin to stain the vascular smooth muscle cells only. 
> I am not just a beginner of histotechnology, but also an
> outsider. However, as a student, I hope I can learn more and practice
> more.
> Yours,
> Circle Chan

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