RE: Antigen Retrieval Methods
|From:||"Smith, Susie" <SSmith@cytologix.com>|
It might be worth your while to attempt antigen retrieval at a pH of 10 or
11 in an electric pressure cooker. You could also try the "double whammy"
(antigen retrieval and enzyme digestion(protease or trypsin)). If this
fails then the tissue is problably beyond resuscitation.
Susie Smith, HT/HTL(ASCP), B.S.
From: Daniel Martinez [mailto:email@example.com]
Sent: Tuesday, November 07, 2000 9:45 AM
Subject: Antigen Retrieval Methods
I currently have a rare Parkinson's case that I
am having trouble staining. This tissue has been
fixing in formalin for 13yrs. I am attempting to
stain this tissue with several alpha-synuclein
antibodies that we produce. To date, I have tried
formic acid, proteinase-K, microwave, and boiling
treatments. Any advice on other methods that might be
worth trying would be appreciated. Thanks for your
CNDR/University of Pennsylvania
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