RE: Antigen Retrieval Methods

From:"Smith, Susie" <>

It might be worth your while to attempt antigen retrieval at a pH of 10 or
11 in an electric pressure cooker.  You could also try the "double whammy"
(antigen retrieval and enzyme digestion(protease or trypsin)).  If this
fails then the tissue is problably beyond resuscitation. 

Susie Smith, HT/HTL(ASCP), B.S.
Research Associate
Cytologix Corporation

-----Original Message-----
From: Daniel Martinez []
Sent: Tuesday, November 07, 2000 9:45 AM
Subject: Antigen Retrieval Methods

     I currently have a rare Parkinson's case that I
am having trouble staining.  This tissue has been
fixing in formalin for 13yrs.  I am attempting to
stain this tissue with several alpha-synuclein
antibodies that we produce.  To date, I have tried
formic acid, proteinase-K, microwave, and boiling
treatments.  Any advice on other methods that might be
worth trying would be appreciated.  Thanks for your

Dan Martinez
CNDR/University of Pennsylvania  

Do You Yahoo!?
Thousands of Stores.  Millions of Products.  All in one Place.

<< Previous Message | Next Message >>