RE: Antigen Retrieval Methods
From: | Patsy.Ruegg@UCHSC.edu |
I would try pepsin at 37dc, about 3 changes for 10 min. each change. I buy
very good premade pepsin from Research Genetics Cat. No 750102.
Patsy
-----Original Message-----
From: Daniel Martinez [mailto:nativenewjersian@yahoo.com]
Sent: Tuesday, November 07, 2000 7:45 AM
To: histonet@pathology.swmed.edu
Subject: Antigen Retrieval Methods
I currently have a rare Parkinson's case that I
am having trouble staining. This tissue has been
fixing in formalin for 13yrs. I am attempting to
stain this tissue with several alpha-synuclein
antibodies that we produce. To date, I have tried
formic acid, proteinase-K, microwave, and boiling
treatments. Any advice on other methods that might be
worth trying would be appreciated. Thanks for your
help
Dan Martinez
CNDR/University of Pennsylvania
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