Questions on IHC

From:"Circle :>" <>

Dear histoners,

I wanna ask some procedures of IHC on aging rat brain  and
aorta. After isolation, I cold the tissue down above the liquid
nitrogen. After freezing, I put them in -70^C. I wanna stain them with
iNOS. And secondly, I want to visualize the endothlial cells on the aorta.

What should I do? I read through many IHC books and my friends
who works with IHC have different protocol. However, as a new one in IHC,
I don't know what is the best way to do.

Thank you very much!


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