Paraffin Block preparation from Cultured Cells

From:Carrie Eberhardt <>

Hello!  I am attempting to make paraffin blocks of cultured cells and our
pathologist has noted that the cell density is not good enough, so I am
asking for tips/ protocols that anyone may be using.

I start with at least 6 million cells, pellet them in a 15 ml centrifuge
tube, pour off media and add a couple drops of warm 3% Eosin/Agar and vortex
to mix.  The cells are immediately chilled on ice and the agar cell pellet
is fixed in Formalin and processed on a short cycle on an automated
processor.  At this time, we embed the entire processed pellet, but we are
trying to core a paraffin block and insert a cored agar pellet with no

Any tips out there?


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