Microwave Processing
From: | Cheryl Crowder <ccrowder@mail.vetmed.lsu.edu> |
Processing tissues with microwaves does require a microwave oven
manufactured for laboratories. There are 3 to my knowledge on the market
today - Milestone, Pella and Energy Beam (which I think now may be marketed
under the TBS brand - but they may be 2 different instruments).
Processing takes different timing, obviously, because of the quickness of
the technique but you can use the reagents you are using now. I have been
trying MW techniques for over 20 years and although I can process tissues
in a kitchen MW, it is hazardous because of the fumes and it is difficult
to regular the temperatures. Each manufacturer has his own protocol for
the procedures. It should be noted that needle biopsies and other small
tissues can be processed very quickly but larger pieces still take 1.5 - 2
hours. Most automatic tissue processors can be programmed for this short
cycle.
If you have any questions or want more information, it would probably be
better to contact me personally by e-mail or phone. I will try to answer
any questions you have.
Cheryl Crowder
Chief Technologist
Department of Veterinary Pathology
School of Veterinary Medicine
Louisiana State University
Baton Rouge, LA 70803
(225) 346-3399
FAX (225) 346-3226
ccrowder@mail.vetmed.lsu.edu
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